Journal of Chromatography & Separation Techniques
Open Access

Monica Whitmire

Publications

• Research Article
  Full Validation of a High Resolution ICP-MS Bioanalysis Method for Iron in Human Plasma with K2EDTA
  Author(s): Monica Whitmire, Anastasia Osredkar, Jennifer Ammerman, Jeff Biss, Chaoyang Huang, Tracy Marshall, Krista Ehringer and Patricia de LisioMonica Whitmire, Anastasia Osredkar, Jennifer Ammerman, Jeff Biss, Chaoyang Huang, Tracy Marshall, Krista Ehringer and Patricia de Lisio
  
  Purpose: To validate a bioanalytical inductively coupled plasma mass spectrometry (ICP-MS) method for quantification of iron in human plasma with K2EDTA (matrix). This method was validated using typical bioanalytical validation parameters. Materials and methods: Iron reference standards and germanium internal standard (IS) were from Ultra Scientific. Reagent water had a resistivity ?18 M?cm. Nitric acid and hydrochloric acid were ultra pure grade. Blank matrix was purchased from BioReclamation. Linearity was assessed using eight standards prepared in proxy matrix over a range equivalent to 0.5-20 ?g iron/mL of matrix at the beginning and end of each run. Quality control (QC) samples were prepared by fortifying matrix at 0.5, 1.5, 10, 15 and 100 ?g/mL. A blank containing IS and a double blank were included in every run. Selectivity was evaluated using six different lots of matrix for.. View More»
  DOI: 10.4172/2157-7064.S4-001

  Abstract PDF

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