Translational Medicine
Open Access
ISSN: 2161-1025
ISSN: 2161-1025
Montaha Lakkis
Siloam Biosciences, Inc.,
Cincinnati
Tanzania
Research Article
Novel Biomarkers Detection and Identification by Microfluidic- Based MicroELISA
Author(s): Montaha Lakkis, Junhai Kai, Nelson Santiago, Aniruddha Puntambekar,Victor Moore, Se Hwan Lee, David W Sehy, Ron Schultheis, Jungyoup Han and Chong H AhnMontaha Lakkis, Junhai Kai, Nelson Santiago, Aniruddha Puntambekar,Victor Moore, Se Hwan Lee, David W Sehy, Ron Schultheis, Jungyoup Han and Chong H Ahn
Miniaturized, high throughput detection technologies including microfluidics systems represent powerful tools for biomarker discovery and analysis. Optimiser™ microplate technology combines microfluidics technology with standard SBS-configured 96-well microplate architecture and allows for the improvement of ELISA workflows. In this review, we present the “standard” and “high sensitivity” capabilities of the Optimiser™ ELISA in detecting human cytokine biomarker; IL-4, resulting in improving sensitivity by 1000-fold higher than the typical conventional ELISA. The Optimiser™ ELISA microplate employs standard ELISA equipment and protocols, selectively reduces sample volume 10-20 fold (Static Mode) and has the capability to amplify assay sensitivity by 1,000 fold (Repetitive Loading Flow-Through Mode) relative to conventional high sensitivity ELI.. View More»
DOI:
10.4172/2161-1025.S1-006