Journal of Chromatography & Separation Techniques
Open Access

Muhammad Saeed Arayne

Publications

• Research Article
  Liquid Chromatographic Analysis of Prazosin in API, Dosage Form and Serum: Application to Drug-Metal Interaction Studies
  
  Author(s): Najma Sultana, Muhammad Saeed Arayne and Shabana Naz Shah Najma Sultana, Muhammad Saeed Arayne and Shabana Naz Shah
  
  Prazosin hydrochloride is a sympatholytic alpha-adrenergic blocker used in the treatment of anxiety, hypertension, refractory pulmonary oedema and panic disorders. Rapid, efficient, cost effective and reproducible isocratic reversed phase method has been developed and validated for the determination of prazosin in active pharmaceutical ingredient, dosage formulation and human serum using mobile phase 75:25 v/v acetonitrile:water having pH 3.20 adjusted with glacial acetic acid. Mobile phase was pumped at a flow rate of 1.5 mL min−1 using gradient elution through prepacked Nuclosil, C18 (250×4.6 mm, 10 μm) column. UV detection was performed at 250 nm. Method was validated following the ICH guidelines. Calibration curve was linear in concentration range 1.0-10 μg mL−1 with correlation coefficient 0.9999, and lower limits of detection and quantitation as 3.3 and 2.. View More»
  DOI: 10.4172/2157-7064.1000197

  Abstract

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