Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Munther Alomari
Australia
Research Article
Profiling the Lipid Raft Proteome from Human MEC1 Chronic Lymphocytic Leukemia Cells
Author(s): Munther Alomari, Swetlana Mactier, Kimberley L. Kaufman, O. Giles Best, Stephen P. Mulligan and Richard I. ChristophersonMunther Alomari, Swetlana Mactier, Kimberley L. Kaufman, O. Giles Best, Stephen P. Mulligan and Richard I. Christopherson
Lipid rafts are specialized micro-domains located in the outer plasma membrane of cells and play important roles in various cellular functions, including cell signalling, secretory and endocytic pathways. Cell surface profiling, in particular the lipid raft proteome, has attracted interest in oncology due to the potential use of raft proteins as novel targets for diagnostics and therapeutics. Three different methods have been used to identify the lipid raft proteome from the human chronic lymphocytic leukemia (CLL) cell line MEC1. Firstly, lipid raft proteins were enriched and identified using sucrose gradient ultracentrifugation and 2D liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). To confirm protein localization to the lipid raft, proteomes were compared before and after cholesterol depletion by methyl-β-cyclodextrin (MβCD) using isobaric tags for re.. View More»
DOI:
10.4172/jpb.S7-005