Journal of Hematology & Thromboembolic Diseases
Open Access

Naiel Azzam

Publications

• Research Article
  An Innovative Cis-Diammineplatinum (II) Benzene-Polycarboxylic-Acids Complex Inhibits Growth of Burkitt`s Cell Lymphoma In Vitro and In Vivo
  Author(s): Lindkær-Jensen S, Jensen CL, Azzam N, Bar-Shalom R, Fares B, et al. Lindkær-Jensen S, Jensen CL, Azzam N, Bar-Shalom R, Fares B, et al.
  
  Cis-diammineplatinum (II) benzene-polycarboxylic acids (CDBPA) is a new therapy of solid tumors without toxicity, mild and transient side-effects and an ability to improve quality of life. The underlying mechanism of action of CDBPA in Burkitt`s lymphoma was tested in vitro and its effect on tumor size and survival was tested in a NOD-scid mice model. BL cells and normal fibroblasts were exposed to CDBPA for cytotoxicity screening by XTT assay, followed by cell cycle arrest, determination of apoptosis using Flow Cytometry and by annexin V/FITC/PI assay. Protein level and Gene expression were tested by Western blotting analysis and by the Applied Biosystems® Tagman® Array Plates, respectively. We demonstrated that CDBPA significantly reduced cell viability and induced apoptosis via the extrinsic and the intrinsic apoptotic pathways of Burkitts lymphoma cells without causing cell cycle arr.. View More»
  DOI: 10.4172/2329-8790.1000234

  Abstract PDF

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