Advanced Techniques in Biology & Medicine
Open Access

Najme Javdani Khalife

Publications

• Research Article
  Development of Gold Nanoparticle Based Colorimetric Biosensor for Detection of Fibronectin in Lung Cancer Cell Line
  Author(s): Reza Nekouian, Najme Javdani Khalife, Zahra Salehi Reza Nekouian, Najme Javdani Khalife, Zahra Salehi
  
  Specific protein detection by means of antibody-nanoparticle conjugates is a new field in medical nanobiotechnology. Among many nanoparticles used, gold nanoparticles show strong light-absorption properties which have been exploited in designing nanobiosensors. Fibronectin (FN) plays an important role in extracellular matrix (ECM) structure and function of normal cells; however, in conditions like lung carcinoma its expression increases, especially in non-small cell lung carcinoma (NSCLC). In this study, we conjugated gold nanoparticles to human fibronectin antibody (anti-hFN) to design a colorimetric nanobiosensor for detection of FN present in ECM of cultured cells. Three different cell lines namely A549 (target cells), AGO-1522 (control cells) and Nalm-6 (negative control cells) were used to compare changes in color resulting from aggregation of gold nanoparticles due to higher amo.. View More»
  DOI: 10.4172/2379-1764.1000118

  Abstract PDF

Relevant Topics