Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Research Article
Inhibitor Design for VacA Toxin of Helicobacter pylori
Author(s): Pasala Chiranjeevi, Sandeep Swargam, Natarajan Pradeep, Kanipakam Hema, Katari Sudheer Kumar, Nalamolu Ravina Madhulitha and Amineni UmamaheswariPasala Chiranjeevi, Sandeep Swargam, Natarajan Pradeep, Kanipakam Hema, Katari Sudheer Kumar, Nalamolu Ravina Madhulitha and Amineni Umamaheswari
Vacuolating cytotoxin autotransporter is secreted by Helicobacter pylori and considered to be an important unique virulence factor in the pathogenesis. VacA toxin is a 140 kDa protoxin contains 1287 amino acids, undergoes N- and C-terminal cleavage to yield a mature 88 kDa toxin consisting of 1-821 residues (p88). Further, matured toxin is crucial to function as active VacA and adheres onto plasma membrane to form anionic channels lead to pathogen entry, formation of vacuoles apt for making colonies in gastric mucosa of humans. As a result, the chronic sequelae such as intolerable gastritis, peptic ulcers, MALT-lymphoma and adenocarcinoma were caused. VacA toxin has attained great attention and selected as drug target to design potential inhibitors. Crystal structure of p55 domain of VacA was prepared and minimized using protein preparation wizard in Maestro v9.8. A grid was generated.. View More»
DOI:
10.4172/jpb.1000409