Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Nguyen Bich Nhi
Vietnam
Research Article
Identification and Characterization of Membrane Proteins from Mouse
Brain Tissue
Author(s): Nguyen Tien Dung, Do Huu Chi, Le Thi Bich Thao, Nguyen Thi Kim Dung, Nguyen Bich Nhi and Phan Van Chi
Nguyen Tien Dung, Do Huu Chi, Le Thi Bich Thao, Nguyen Thi Kim Dung, Nguyen Bich Nhi and Phan Van Chi
Membrane proteins are attractive targets for proteomics research because of their vital roles in numerous processes of the cell including: cell adhesion, immune response, metabolism and signal transduction... At the start of proteomics research, two-dimensional gel electrophoresis (2D-PAGE) was routinely used to separate complex proteomic samples. However, this method faces difficulties in separating membrane proteins due to their hydrophobicity. In this study, mouse brain membrane fractions were prepared using carbonate extraction and ultracentrifugation. The separation and identification of the membrane proteins by using a gel-based approach in combination with two-dimensional nano liquid chromatography coupled online with tandem mass spectrometry (2DNanoLC-Q-TOF-MS/MS) were presented. In total, 298 identified membrane proteins from mouse brain tiss.. View More»
DOI:
10.4172/jpb.1000272