Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Nikonov SD
Serbia
Research Article
Low Antigen-specific T-cell Response in Pulmonary Tuberculosis is Associated with Impaired Phenotype and Functions of Interferon-α Induced Dendritic Cells
Author(s): Sakhno LV, Leplina OYu, Tikhonova MA, Shevela EYa, Nikonov SD, Zhdanov OA, Ostanin AA and Chernykh ERSakhno LV, Leplina OYu, Tikhonova MA, Shevela EYa, Nikonov SD, Zhdanov OA, Ostanin AA and Chernykh ER
The phenotype and functions of monocyte-derived dendritic cells (DCs), generated upon stimulation with GMCSF and IFN-α, were investigated in pulmonary tuberculosis (TB). Patient INF-DC cultures were characterized by increased number of CD14+ cells, lowered level of CD25+ cells and increased expression of B7-H1. Besides, DCs produced enhanced levels of IL-6 and IL-10 and differed by reduced secretion of INF-γ and INF-α. Patient IFN-DCs had lower capacity to stimulate allogeneic T-cell proliferation and induce CD3+IFN-γ+ T cells but increased ability to activate CD3+IL-4+ T cells in mixed lymphocyte culture. The most pronounced increase of IL-10 production, as well as the decrease of allostimulatory activity and the alteration of T1/T2 stimulatory activity of DCs were registered in patients with low PP.. View More»
DOI:
10.4172/2155-9899.1000169