Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Noa Cohen
Tanzania
Research Article
T Cell Dynamic Activation and Functional Analysis in Nanoliter Droplet Microarray
Author(s): Saheli Sarkar, Vinny Motwani, Pooja Sabhachandani, Noa Cohen and Tania KonrySaheli Sarkar, Vinny Motwani, Pooja Sabhachandani, Noa Cohen and Tania Konry
Objective: Characterization of the heterogeneity in immune reactions requires assessing dynamic single cell responses as well as interactions between the various immune cell subsets. Maturation and activation of effector cells is regulated by cell contact-dependent and soluble factor-mediated paracrine signalling. Currently there are few methods available that allow dynamic investigation of both processes simultaneously without physically constraining non-adherent cells and eliminating crosstalk from neighboring cell pairs. We describe here a microfluidic droplet microarray platform that permits rapid functional analysis of single cell responses and co-encapsulation of heterotypic cell pairs, thereby allowing us to evaluate the dynamic activation state of primary T cells. Methods: The microfluidic droplet platform en.. View More»
DOI:
10.4172/2155-9899.1000334