Medicinal & Aromatic Plants
Open Access

NORDINE Aicha

Publications

• Research Article
  Rapid in Vitro Regeneration and Clonal Multiplication of Thymus Bleicherianus Pomel, a Rare and Threatened Medicinal and Aromatic Plant in Morocco
  Author(s): NORDINE Aicha and EL MESKAOUI AbdelmalekNORDINE Aicha and EL MESKAOUI Abdelmalek
  
  This paper reports, for the first time, an efficient and reliable protocol for in vitro clonal propagation of T. bleicherianus, a rare aromatic and medicinal plant in Morocco. Initially, nodal explants collected from wild plant were used for in vitro culture establishment on Murashige and Skoog (MS) basal salts medium without plant growth regulators (PGR). Browning and contaminants were the major obstacles for in vitro culture establishment using explant derived from wild plants. To overcome this problem, the use of polyvinylpyrrolidone together with dark treatment decreased browning from 100 to 25%. The aseptic and living explants were then transferred into MS medium and all cultures were placed under light conditions. The cytokinin type and concentration were the most important factors affecting shoot multiplication. Axillary shoot bud proliferation was achieved using plant growth r.. View More»
  DOI: 10.4172/2167-0412.1000145

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