Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

Olivia C Glass

Publications

• Research Article
  Cryorecovery of Mouse Sperm by Different IVF Methods Using MBCD and GSH
  Author(s): Ming-Wen Li, Olivia C Glass, Jasmin Zarrabi, Lisa N. Baker and K. C. Kent Lloyd Ming-Wen Li, Olivia C Glass, Jasmin Zarrabi, Lisa N. Baker and K. C. Kent Lloyd
  
  Different protocols incorporating methyl-β-cyclodextrin (MBCD) and reduced glutathione (GSH) have been reported to improve IVF recovery of cryopreserved mouse sperm on a C57BL/6 (J and N) genetic background. However, it is not clear which IVF protocol is most appropriate when using the various methods to cryorecover sperm with different sperm quality and sample volumes. Therefore, in the present study we correlated sperm motility with fertilization rate and compared the efficiency of different IVF methods using various sperm samples so as to establish general guidelines for mouse sperm cryorecovery by IVF. High linear correlation between sperm fertilization rate and progressive motility was found, R2 was 0.9623 and 0.9993 for pre-freezing and post-thaw progressive motility, respectively. High amounts of cryoprotective agent (CPA) were observed to impair both sperm capacitation an.. View More»
  DOI: 10.4172/2375-4508.1000175

  Abstract PDF

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