Journal of Proteomics & Bioinformatics
Open Access

Pablo HCG de Sa

Publications

• Research Article
  Efficiency of Corynebacterium pseudotuberculosis Cp31 Genome Assembly with the Hi-Q Enzyme on an Ion Torrent PGM Sequencing Platform
  Author(s): Adonney AO Veras, Pablo HCG de Sa, Kenny C Pinheiro, Diego Assis das Gracas, Rafael Azevedo Barauna, Maria Paula Cruz Schneider, Vasco Azevedo, Rommel TJ Ramos and Artur SilvaAdonney AO Veras, Pablo HCG de Sa, Kenny C Pinheiro, Diego Assis das Gracas, Rafael Azevedo Barauna, Maria Paula Cruz Schneider, Vasco Azevedo, Rommel TJ Ramos and Artur Silva
  
  Despite the high accuracy obtained through high throughput sequencing (HTS) platforms, including sequencing methods such as pyrosequencing (Roche 454), ligase (SOLiD System), and recently post-light sequencing using the Ion Torrent PGM that is able to detect ions released during sequencing, there are still many errors inherent in the chemistry used by these platforms, for example, INDEL (Insertion/Deletion) that are very common on platforms 454 of Roche and Ion Torrent PGM; Substitution abound in Illumina platforms and SOLiD. Thus, efforts to address these problems have been undertaken by the sequencing companies. To improve the accuracy of the Ion Torrent PGM reads, Life Technologies has developed an enzyme called Hi-Q. This work aims to demonstrate the performance of the genome assembly of Corynebacterium pseudotuberculosis Cp31 using the enzyme Hi-Q. To evaluate the results.. View More»
  DOI: 10.4172/jpb.1000342

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