Journal of Proteomics & Bioinformatics
Open Access

Pengyuan Yang

Publications

• Research Article
  Development of Core-shell Magnetic Mesoporous SiO2 Microspheres for the Immobilization of Trypsin for Fast Protein Digestion
  Author(s): Dawei Qi, Yonghui Deng, Yingchao Liu, Huaqing Lin, Chunhui Deng, Yan Li, Xiangmin Zhang, Pengyuan Yang and Dongyuan Zhao Dawei Qi, Yonghui Deng, Yingchao Liu, Huaqing Lin, Chunhui Deng, Yan Li, Xiangmin Zhang, Pengyuan Yang and Dongyuan Zhao
  
  In the work, we developed glycidoxypropyltrimethoxysilane (GLYMO)-modified Fe3O4@SiO2 core and perpendicularly aligned mesoporous SiO2 shell (designated Fe3O4@nSiO2@mSiO2) as the novel substrate for the immobilization of large amount of trypsin and applied it for fast protein digestion. Firstly, Fe3O4@nSiO2@mSiO2 microspheres were synthesized. Then, the surface of the microspheres was functionalized with GLYMO for enzyme immobilization.The amount of trypsin immobilized on Fe3O4@nSiO2@mSiO2 was about 188 ? g/mg, which was much more than that on the previous magnetic materials. Using the trypsin-immobilized magnetic mesoporous SiO2 microspheres, proteins in samples were fast digested with microwave irradiation. The efficacy of this technique for protein mapping was demonstrated by the mass spectral analysis of the peptide fragmentation of three standard proteins, includ.. View More»
  DOI: 10.4172/jpb.1000043

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