Chemotherapy: Open Access
Open Access
ISSN: 2167-7700
ISSN: 2167-7700
Ping Lan
China
Research Article
A Novel Strategy to Identify mRNA 3′ UTR and mRNA Level Difference in Crohn’s Disease
Author(s): Tuo Hu, Hua-shan Liu, Chi Zhou, Xian-rui Wu, Yu-feng Chen, Xuan-hui Liu, Xiao-wen He, Yi-feng Zou, Xiao-sheng He, Xiao-jian Wu, Ping Lan and Jia KeTuo Hu, Hua-shan Liu, Chi Zhou, Xian-rui Wu, Yu-feng Chen, Xuan-hui Liu, Xiao-wen He, Yi-feng Zou, Xiao-sheng He, Xiao-jian Wu, Ping Lan and Jia Ke
Abstract Background and Aims: Crohn’s disease (CD) is a chronic and refractory intestinal inflammatory disease. The 3′ untranslated region (3′ UTR) of mRNA transcript can regulate its own translational process post-transciptionally, and its role in CD remains unclear. The aim of this study was to identify the critical genes influencing CD phenotype via the regulation of mRNA 3′ UTR. Methods: Isoform Structural Change Model (IsoSCM) was used to evaluate the length of 3′ UTR of the whole transciptome from a RNA-seq based online CD database. Correlations between 3′ UTR length status and mRNA level were analyzed by Spearman coefficients. Correlated genes list was overlapped with published critical CD related gene list. Univariate and multivariate analysis were performed to identify the genes associated with CD phenotype. Results: Compared with normal co.. View More»
DOI:
10.4172/2167-7700.1000226