Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Pinpin Lin
National Health Research Institutes,
No. 35 Keyan Road, Zhunan Town, Miaoli County 350
People's Republic of China
Research Article
Proteomics Investigation Reveals Apoptosis-Associated Proteins in Aryl Hydrocarbon Receptor-Deficient Human Lung Cells Treated with 2,3,7,8-Tetrachlorobenzo-p-dioxin
Author(s): Eric S. L. Hsiao, Yin-Wei Chang, Pinpin Lin and Pao-Chi LiaoEric S. L. Hsiao, Yin-Wei Chang, Pinpin Lin and Pao-Chi Liao
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), the most potent dioxin, binds to aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor, which subsequently alters gene expression. Epidemiological studies suggested that exposure to TCDD highly enhanced the risk of pulmonary diseases. In this study, we adopted a comparative proteomic approach using two-dimensional gel electrophoresis (2DE) to identify the differential expression of proteins between TCDD-treated control and AhR-deficient lung adenocarcinoma cells. Fifteen proteins from seventeen differential protein spots were identified that four of which were down-regulated and eleven were up-regulated. Western blotting analysis confirmed differential expression for five identified proteins, including GRB10, YWHAG, HSP27, LGALS1 and ACTB. Further protein-protein interaction network analysis of the identified proteins illu.. View More»
DOI:
10.4172/jpb.1000208