Journal of Probiotics & Health
Open Access
ISSN: 2329-8901
ISSN: 2329-8901
Research Article
Lactobacillus and Bifidobacterium Promote Antibacterial and Antiviral Immune Response in Human Macrophages
Author(s): Davies TS, Plummer SF, Jack AA, Allen MD and Michael DR
Davies TS, Plummer SF, Jack AA, Allen MD and Michael DR
Objective: The objective of this study was to identify the immunomodulatory potential of two consortia of lactic acid bacteria, “Lab4” and “Lab4b”, using human macrophages as an in vitro model system.
Methods: THP-1 monocyte-derived macrophages were exposed to metabolites of Lab4 or Lab4b. RT-qPCR was performed on macrophages to determine the expression of M1 pro-inflammatory (IL-1β, IL-18 and CD80) or M2 anti-inflammatory (CD206) marker mRNA in addition to IL-1β protein and inflammasome (NLRP3, Caspase-1, NLRP1, NLRC4 and AIM2) mRNA expression. Bacterial (LPS and ATP) and viral (Poly I:C) challenge were stimulated to determine the potential of these consortia to regulate IL-1β protein, inflammasome mRNA expression, and antiviral IL-12 mRNA expression and protein under inflammatory conditions. The ability .. View More»
DOI:
10.4172/2329-8901.1000195