Journal of Chromatography & Separation Techniques
Open Access

Pradip N. Bhoya

Publications

• Research Article
  Simultaneous Estimation of Tramadol Hcl, Paracetamol and Domperidone in Pharmaceutical Formulation by Thin-Layer Chromatographic Densitometric Method
  Author(s): Pradip N. Bhoya, Emanual M. Patelia and Guatambhai Pradip N. Bhoya, Emanual M. Patelia and Guatambhai
  
  A simple, precise, rapid, selective, and economic high-performance-thin-layer chromatography (HPTLC) method has been established for simultaneous analysis of Domperidone (DMP), Paracetamol (PCM) and Tramadol Hcl (TMD) in tablet dosage forms. The chromatographic separations were performed on precoated silica gel 60254 plates with toluene-ethylacetate-butanol-ammonia 5:4:1:0.2(v/v) as mobile phase. The plates were developed in a 7.0 cm at ambient temperature. The developed plates were scanned and quantified at their single wavelength of maximum absorption at approximately 278 nm for DMP and PCM, respectively. Experimental conditions such as chamber size, chamber saturation time, migration of solvent front, slit width, etc. was critically studied and the optimum conditions were selected. The drugs were satisfactorily resolved with Rf 0.18 ± 0.02 for DMP, Rf 0.25 ± 0.02 for .. View More»
  DOI: 10.4172/2157-7064.1000139

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