Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Prajakta Shahir
India
Research Article
Early IgM Antibody Response in Chandipura Virus Infection: T cell-Independent Activation of B-cells
Author(s): Anukumar Balakrishnan and Prajakta ShahirAnukumar Balakrishnan and Prajakta Shahir
The T cell-independent B-cells activation by Chandipura virus is not known. In the current study, T cellindependent activation of B-cells by Chandipura virus was studied. The activation of B-cells was studied in the experiments determining the membrane expression of CD25 and CD69 markers, proliferation and virus specific IgM antibody secretion. Activation and proliferation of B-cells, and IgM secretions could be observed from the cells exposed to live virus. In case of B-cells treated with inactivated virus proliferation and IgM secretion was not observed. In order to determine the role of TLR4 in B-cell activation, the membrane TLR4 on B-cells were blocked with anti TLR4/MD2 antibody and exposed to live virus. Like inactivated virus, the antibody blocked cells were not showed proliferation and IgM secretion. In in vivo mice experiment, the T cell deficient nude mice were exp.. View More»
DOI:
10.4172/2155-9899.1000123