Fungal Genomics & Biology
Open Access
ISSN: 2165-8056
ISSN: 2165-8056
Raheleh Dehgahi
School of Biological Sciences,
Georgetown
Malaysia
Research Article
Gamma Irradiation-Induced Variation in Dendrobium Sonia-28 OrchidProtocorm-Like Bodies (PLBs)
Author(s): Raheleh Dehgahi* and Alireza JoniyasaRaheleh Dehgahi* and Alireza Joniyasa
Dendrobium sonia-28 is an important ornamental orchid in the flower industry. In vitro mutagenesis by means of gamma irradiation can be used to produce economically improved mutants. Protocorm-like bodies (PLBs) of Dendrobium sonia-28 were subjected to gamma irradiation at doses of 10 to 200 Gy gamma rays. Results showed that PLBs survival rate and weight were inversely related to the irradiation doses. In untreated PLBs, survival rate and weight were tenfold and fivefold higher as compared to 200 irradiated PLBs. Results indicated that the radio sensitivity test (LD50) for the PLBs were approximately at 43 Gy. There were appreciable variations in plantlet structure and morphology relative to increase irradiation doses. Plantlets infected with low doses of gamma radiation had better development of the shoot and root as well as the foliage. SEM and TEM analysis disclosed sever.. View More»
DOI:
10.4172/2165-8056.1000151