ISSN: 2329-9029
+44 1478 350008
Raj Kiran Bala
Central Research Lab, Department of Reseach and Development, Sree Balaji Medical College and Hospital,
Chennai
India
Research Article
Identification and Characterization of Novel Auxin Biosynthetic Mutants
Author(s): Raj Kiran Bala
Raj Kiran Bala
Indole-3-acetic acid (IAA) is the primary form of auxin in plants and several IAA biosynthetic pathways have been previously proposed but remain genetically uncharacterized. One of the existing pathways is the indole-3-pyruvic acid (IPyA) pathway, which is known to regulate key developmental processes such as apical hook formation and shade avoidance. Recent studies suggest the existence of the pathway in higher plants but are unverified due to the elusive nature of IPyA in vitro. Extending on these recent advances, this research was aimed at investigating aspects of IPyA-dependent auxin biology in Pisum sativum (pea) using reverse genetics. Consequently, using a reverse genetic approach, called TILLING, the PsTAR2 gene was mutated in order to study firsthand the downstream effects of IPyA deficiency.
The procedure resulted in isolating two novel PsTAR2 (IPyA) mutant lines c.. View More»
DOI:
10.4172/2329-9029.1000212