ISSN: 2167-0412
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Raja M
India
Research Article
In vitro Antimicrobial Activity of Azadirachta indica (Leaves) against Fish
Pathogenic Bacteria Isolated from Naturally Infected Dawkinsia
filamentosa (Blackspot barb)
Author(s): Kavitha M, Raja M, Kamaraj C, Karthik Raja R, Balasubramaniam V, Balasubramani G and Perumal P
Kavitha M, Raja M, Kamaraj C, Karthik Raja R, Balasubramaniam V, Balasubramani G and Perumal P
To examine the antibacterial activity of ethyl acetate, ethanol and methanol extracts of Azadirachta indica (A. indica) on fish pathogens viz., Aeromonas veronii, Aeromonas hydrophila, Acinetobacter junii, Acinetobacter tandoii, Acinetobacter spp. and Pseudomonas stutzeri isolated from diseased blackspot barb, Dawkinsia filamentosa. The naturally infected fish, D. filamentosa were collected from Mettur Dam and the associated bacteria in the ulcerative lesions were isolated, sequenced the amplified product and tentatively identified through sequence similarity. The leaves powder was extracted successively with 250 ml of ethyl acetate, ethanol and methanol solvents by using Soxhlet extractor for 8 h at a temperature not exceeding the boiling point of the solvent. Ethyl acetate, ethanol and methanol extracts were subjected to phytochemical analysis as well as the antimicrobial assay usin.. View More»
DOI:
10.4172/2167-0412.1000294