Mycobacterial Diseases
Open Access
ISSN: 2161-1068
+44 1478 350008
ISSN: 2161-1068
+44 1478 350008
Rajaa Al-Attiyah
Kuwait
Research Article
The Natural Expression of Genes Encoding Major Antigens of Rd1 and Rd9 in M. Tuberculosis and other Mycobacteria
Author(s): Shumaila N. M. Hanif, Rajaa Al-Attiyah and Abu Salim MustafaShumaila N. M. Hanif, Rajaa Al-Attiyah and Abu Salim Mustafa
The natural expression of genes of five major antigenic proteins present in the genomic region of difference (RD)1, i.e. pe35, ppeE68, esxA and esxB , and RD9, i.e. esxV , was determined, at mRNA and protein levels, in M. tuberculosis, M. bovis BCG, and environmental mycobacterial species of M.avium and M.vaccae . The expression of mRNA was determined using total mycobacterial RNA and gene-specific forward and reverse primers in reverse transcription-polymerase chain reaction (RT-PCR), and the expression of proteins was determined by Western immunoblot analysis of mycobacterial sonicates with anti-sera containing protein-specific antibodies raised in rabbits. Furthermore, the expression of each protein in various sub-cellular fractions of M.tuberculosis was studied by Western immunoblot analysis of cell walls, culture filtrates and sonicates of M... View More»
DOI:
10.4172/2161-1068.1000105