Journal of Glycobiology
Open Access

Rajagopal Chattopadhyaya

Publications

• Research Article
  Mannose in Complex with Colocasia esculenta Tuber Agglutinin
  Author(s): Rajagopal Chattopadhyaya and Avisek MondalRajagopal Chattopadhyaya and Avisek Mondal
  
  The major tuber storage protein of Colocasia esculenta is a monocot mannose-binding, widely used, dietarylectin, for which a crystal structure was previously shown to consist of four β-prism II domains or two αβheterodimers, each forming a α2β2 heterotetramer with a symmetry related unit. In the present study, the same lectincrystallized in the presence of a 50 molar excess of free mannose, persists in forming such α2β2 heterotetramers.Lectin crystals apparently complexed to mannose were obtained by hanging-drop, vapor-diffusion method at roomtemperature and high-resolution X-ray diffraction data were collected using a home X-ray source. Using the crystalstructure of the Remusatia vivipara lectin, the structure of the complex has been solved by molecular replacementand subsequent crystallographic refinement. Five different mannose binding sites pe.. View More»
  DOI: 10.4172/2168-958X.1000132

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