Journal of Clinical Toxicology
Open Access

Rajesha Nairy

Publications

• Research Article
  Cytotoxicity Studies of Functionalized Gold Nanoparticles Using Yeast Comet Assay
  Author(s): Saritha Suvarna, Rajesha Nairy, Sunil K C and Narayana Y Saritha Suvarna, Rajesha Nairy, Sunil K C and Narayana Y
  
  In the present study gold nanoparticles and glucose capped gold nanoparticles are synthesized by chemical route method and characterized using UV-SPR, FTIR and TEM analysis. Single cell gel electrophoresis (SCGE) assay was used to study DNA damage. Studies show that glucose capped gold nanoparticles are less toxic as compare to gold nanoparticles at DNA level. Somewhat larger gold nanoparticle used to monitor endocytosis in log-phase S. cervisiae spheroplasts at 10 to 30 μM was not reported to cause growth inhibition. It shows that glucose capped gold nanoparticles are nontoxic to yeast strain D7. DNA damage was observed by using standard method called Yeast comet assay, which provides a very sensitive method for detecting strand breaks and repair kinetics in single cells. Studies showed that 5 μM-30 μM having very less sign of DNA damage in case of Glucose capped gold nanopa.. View More»
  DOI: 10.4172/2161-0495.1000347

  Abstract PDF

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