Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Raju V.S. Rajala
University of Oklahoma Health Sciences Center 608 Stanton L. Young Blvd,
Oklahoma City , OK 73104
Tanzania
Research Article
The Optimized Conditions of Two Dimensional Polyacrylamide Gel Electrophoresis for Serum Proteomics
Author(s): Raju V.S. Rajala
Raju V.S. Rajala
Serum proteome analysis provides a potential promising approach in disease diagnosis and therapeutic monitoring. However, the large dynamic range of proteins, high-abundant proteins, excess of salt and lipid in serum makes the analysis very challenging. Therefore, it is imperative to improve the dissolution of proteins in twodimensional gel electrophoresis (2-DE), and enhance the ability to analyze the proteome of serum under a wide variety of physiological conditions. This study examined the effects of various combinations of depletion highabundant protein, precipitated means, hot SDS treatment and IPG strips with different pH on 2-DE map for mouse serum. Finally the removal of high-abundant proteins in serum by the ProteoExtractTM Albumin Removal column, ethanol precipitation, the heating with 2.5% SDS and 2.3% DTT to denature sample at 95oC for 3 min, and IEF on pH.. View More»
DOI:
10.4172/jpb.1000032