Cell & Developmental Biology
Open Access

Ranjita Sinha

Publications

• Research Article
  Over-expression of Arginine Decarboxylase Gene in Tapetal Tissue Results in Male Sterility in Tomato Plants
  Author(s): Ranjita Sinha and Manchikatla V RajamRanjita Sinha and Manchikatla V Rajam
  
  Homeostasis of cellular Polyamines (PAs) is very important for proper growth and development of living organisms. Development of male sex organ and gametophyte in plants is a complex process involving many known and unknown pathways. PAs are actively involved in the development of anther and pollen. In the present study, oat arginine decarboxylase (ADC) gene has been over-expressed in tapetum of tomato using tapetum-specific TA29 promoter to perturb the PA homeostasis in order to see its implication on male fertility. Tapetum-specific ADC overexpression resulted in PA accumulation, mainly in free and bound fractions in the anther of transgenics. Transgenic lines displayed abnormal pollen and tapetum development. Pollen grains were aborted and distorted in morphology and tapetum showed premature degeneration in ADC transgenics. These transgenics failed to set seeds, but cross pollinati.. View More»
  DOI: 10.4172/2168-9296.1000117

  Abstract PDF

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