Advanced Techniques in Biology & Medicine
Open Access

Raymond RR Rowland

Publications

• Research Article
  Evaluation of Classical Swine Fever Virus (CSFV)-Specific IgA, IgG, and IgM Antibody response in Swine Vaccinated with Alphavirus Replicon Particles-Expressed Antigens
  Author(s): Mohammad M Hossain* and Raymond RR Rowland Mohammad M Hossain* and Raymond RR Rowland
  
  Classical swine fever virus (CSFV) E2 glycoprotein was expressed in an alphavirus based replicon particles (RP) expression system. A fluorescent microsphere immunoassay (FMIA) has been developed for the detection of CSFV E2-specific antibody in swine vaccinated with alphavirus RP. CSFV full length E2 (aa 1-376) was fragmented into several pieces and recombinant proteins were expressed in Escherichia coli. Purified proteins were conjugated to microsphere beads, the target antigens were assembled into a single multiplex, and tested against sera vaccinated with alphavirus-expressed antigens. The results reported as mean fluorescence intensity (MFI) obtained from the median value for at least 100 microspheres and the MFI values converted to positive per sample (S/P) ratio. Of the eight recombinant E2 proteins evaluated in this study, the highest MFI values were obtained for E2 (aa 1-181)... View More»
  DOI: 10.4172/2379-1764.1000213

  Abstract PDF

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