Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
+44 1223 790975
ISSN: 2155-9570
+44 1223 790975
Rebecca M Sappington
The Vanderbilt Eye Institute,
11425 Medical, Research Building IV, Nashville, TN 37232-0654
Tanzania
Research Article
Pressure-Induced Alterations in PEDF and PEDF-R Expression: Implications for Neuroprotective Signaling in Glaucoma
Author(s): Sean J Lee, D’Anne S Duncan, Franklin D Echevarria, William M McLaughlin, Jeremy B Hatcher and Rebecca M SappingtonSean J Lee, D’Anne S Duncan, Franklin D Echevarria, William M McLaughlin, Jeremy B Hatcher and Rebecca M Sappington
Introduction: Alterations in neuron-glia signaling are implicated in glaucoma, a neurodegenerative disease characterized by retinal ganglion cell (RGC) death. Pigment epithelium derived factor (PEDF) is a secreted protein with potential neuroprotective qualities in retinal disease, including chronic ocular hypertension. Here we sought to determine whether moderate, short-term elevations in IOP alter PEDF signaling and whether pressure-induced PEDF signaling directly impacts RGC apoptosis. Methods: In retina from naïve mice and mice with unilateral, microbead-induced glaucoma, we examined expression and cell type-specific localization of PEDF and its receptor (PEDF-R), using quantitative PCR and immunohistochemistry. Using primary cultures of purified RGCs and Müller cells, we examined cell type-specific expression of PEDF in response to 48 hours of elevated hydrostatic press.. View More»
DOI:
10.4172/2155-9570.1000491