Journal of Clinical and Experimental Ophthalmology
Open Access

Rebecca M Sappington

Publications

• Research Article
  Pressure-Induced Alterations in PEDF and PEDF-R Expression: Implications for Neuroprotective Signaling in Glaucoma
  Author(s): Sean J Lee, D’Anne S Duncan, Franklin D Echevarria, William M McLaughlin, Jeremy B Hatcher and Rebecca M SappingtonSean J Lee, D’Anne S Duncan, Franklin D Echevarria, William M McLaughlin, Jeremy B Hatcher and Rebecca M Sappington
  
  Introduction: Alterations in neuron-glia signaling are implicated in glaucoma, a neurodegenerative disease characterized by retinal ganglion cell (RGC) death. Pigment epithelium derived factor (PEDF) is a secreted protein with potential neuroprotective qualities in retinal disease, including chronic ocular hypertension. Here we sought to determine whether moderate, short-term elevations in IOP alter PEDF signaling and whether pressure-induced PEDF signaling directly impacts RGC apoptosis. Methods: In retina from naïve mice and mice with unilateral, microbead-induced glaucoma, we examined expression and cell type-specific localization of PEDF and its receptor (PEDF-R), using quantitative PCR and immunohistochemistry. Using primary cultures of purified RGCs and Müller cells, we examined cell type-specific expression of PEDF in response to 48 hours of elevated hydrostatic press.. View More»
  DOI: 10.4172/2155-9570.1000491

  Abstract PDF

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