Applied Microbiology: Open Access
Open Access

ISSN: 2471-9315

+44 1300 500008

Reddy GV

Reddy GV

India

Publications
  • Research Article
    Microbial Diversity of Ammonia Oxidizing Bacteria through Waste Water Genomics
    Author(s): Maulin Pramod Shah and Reddy GV Maulin Pramod Shah and Reddy GV

    Autotrophic ammonia-oxidizing bacteria are a critical factor of the microbial community in industrial wastewater treatment systems. We evaluated the diversity and community composition of β-proteobacterial ammonia-oxidizing bacteria in two full-scale treatment reactors - a sand filter and a biological aerated filter - receive an identical wastewater. Polymerase chain reaction of the 16S rRNA gene fragments of ammonia-oxidizing bacteria-selective primers was merged with denaturing gradient gel electrophoresis to allow the comparative analysis of the dominant ammonia-oxidizing bacteria populations. The phylogenetic affinities of the dominant ammonia-oxidizing bacteria were verified by cloning and sequencing of polymerase chain reaction-amplified 16S rRNA gene fragments. Denaturing gradient gel electrophoresis profiles were evaluated using a probability-based similarity index. An ex.. View More»
    DOI: 10.4172/2471-9315.1000110

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