ISSN: 0974-276X
Richard A Yost
Tanzania
Research Article
Liquid Chromatography-Mass Spectrometry Metabolic and Lipidomic
Sample Preparation Workflow for Suspension-Cultured Mammalian Cells
using Jurkat T lymphocyte Cells
Author(s): Candice Z Ulmer, Richard A Yost, Jing Chen, Clayton E Mathews and Timothy J GarrettCandice Z Ulmer, Richard A Yost, Jing Chen, Clayton E Mathews and Timothy J Garrett
Metabolomics is the comprehensive study of metabolism as it pertains to an organism or biological system. Lipidomics, a subset discipline of metabolomics, encompasses the study of cellular lipid functions: including pathways, networks, and interactions. The abundance of metabolites and lipids, along with their contribution to health and disease, makes metabolomics a valuable tool for biomarker research. Disease biomarker identification requires a reproducible, sensitive, and accurate analytical platform. Although transcriptomic and proteomic areas have well-established protocols for sample preparation and data processing, the metabolomics field is still developing comparable standardized conventions. Furthermore, of the few comparative LC-MS metabolomic studies that have been applied to mammalian cell cultures, most are targeted to adherent cell lines. The purpose of this work was to .. View More»
DOI:
10.4172/jpb.1000360