Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
RK Garg
Centre of Excellence in Biotechnology, M.P. Council of Science and Technology,
Vigyan Bhawan, Nehru Nagar, Bhopal-462003, Madhya Pradesh
India
Research Article
Mitochondrial COI Gene Sequence Analyses of Puntius ticto and Compared with Seven Species of Genus Puntius of Family Cyprinidae: A Finding for Phylogenetic Positioning and DNA Barcoding as Model Study for Cryptic Species
Author(s): RK Garg, Nidhi Dubey, N Batav, Pooja Pandey and RK Singh
RK Garg, Nidhi Dubey, N Batav, Pooja Pandey and RK Singh
Over the last three decades, mitochondrial DNA (mtDNA) has declared as the most popular marker of molecular diversity, for a combination of technical ease-of-use considerations, and supposed biological and evolutionary properties of a species. The present study examined partial mitochondrial cytochrome c oxidase subunit I gene sequence of mitochondrial DNA for phylogenetic positioning of Puntius ticto among eight species of genus Puntius and its suitability to determine the genetic differentiation in among genus Puntius. The 05 samples of P. ticto were collected from Halali reservoir were analyzed mtcox1 gene of mitochondrial partial regions were sequenced and compared with the online database available on NCBI (National Centre for Biotechnology Information, USA) for rest of seven species (P. sophore, P. sarana, P. amphibious, P. chola, P. conchonius, P. dorsalis and P. gelius). Seque.. View More»
DOI:
10.4172/jpb.1000445