Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Robert D Baker
Tanzania
Research Article
Paraoxonase Gene Expression in Pediatric Inflammatory Bowel Disease
Author(s): Razan H Alkhouri, Susan S Baker, Humaira Hashmi, Wensheng Liu, Robert D Baker and Lixin ZhuRazan H Alkhouri, Susan S Baker, Humaira Hashmi, Wensheng Liu, Robert D Baker and Lixin Zhu
Background: Oxidative stress plays a role in the pathogenesis of inflammatory bowel disease (IBD). The Paraoxonase (PON) genes, expressed in the human intestine, are thought to prevent oxidative stress and modulate inflammation. We investigated the effect of IBD and steroids on PON gene. We hypothesized that PON gene expression is decreased in IBD patients and that steroid treatment would return it to normal.Methods: Pediatric patients diagnosed with IBD, were enrolled and matched to control subjects. For in vitro studies, human epithelial colorectal adenocarcinoma (Caco-2) cells were treated with hydrogen peroxide (H2O2) and dexamethasone. PON genes expression was evaluated by quantitative real-time PCR for both the biopsies and the Caco-2 cells.Results: PON gene expression was decreased in .. View More»
DOI:
10.4172/2155-9899.1000224