Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Roberto Pacelli
Italy
Research Article
A Role for Proteomics in Identifying Targets for Radiosensitizing
Strategies in Melanoma: The FKBP51 Paradigm
Author(s): Anna D'Angelillo, Roberto Pacelli, Rosanna Martinelli, Paolo D'Arrigo, Stefania Staibano, Raffella Avellino, Luigi Sivero, Rita Bisogni and Simona RomanoAnna D'Angelillo, Roberto Pacelli, Rosanna Martinelli, Paolo D'Arrigo, Stefania Staibano, Raffella Avellino, Luigi Sivero, Rita Bisogni and Simona Romano
The treatment of metastatic melanoma is challenging and in the vast majority of cases unsuccessful. Melanoma cells are resistant to most standard therapeutics. We have recently demonstrated that FKBP51 regulates melanoma response to ionizing radiation (IR). To find out molecular targets for radiosensitizing strategies to apply in this neoplasm, we investigated the changes of protein profiles in irradiated melanoma depleted or not of FKBP51, by protein microarray approach. Among the multiple molecules that were found modulated in our cell model, the decrease of several pPKC isoforms in the FKBP51-depleted (IR-sensitive) melanoma appeared to us particularly interesting, because PKC is involved in radiation response. Therefore, PKC was chosen for further investigation. After validating by western blot proteomics results, we found that targeting PKC, with the pan PKC inhibitor LY317615 or.. View More»
DOI:
10.4172/jpb.1000325