Journal of Glycobiology
Open Access

Romildo Albuquerque Nogueira

Publications

• Research Article
  Pro-angiogenic Activity Assay of Chondroitin Sulfate and Glucosamine Sulfate on Vascular Network of Mouse and of Chick Embryo Chorioallantoic Membrane
  Author(s): Fernanda Katharine de Souza Lins Borba1,2, Edbhergue Ventura Lola Costa1, Viviane Aparecida Balvedi Polli2, Daniela Sousa Coelho2, Marcelo Maraschin2, Paulo Fernando Dias2 and Romildo Albuquerque Nogueira1*Fernanda Katharine de Souza Lins Borba1,2, Edbhergue Ventura Lola Costa1, Viviane Aparecida Balvedi Polli2, Daniela Sousa Coelho2, Marcelo Maraschin2, Paulo Fernando Dias2 and Romildo Albuquerque Nogueira1*
  
  Objective: Target of this study was to test the capacity of chondroitin sulfate (CS) and glucosamine sulfate (GS) to induce in vivo angiogenesis. Methods: The proangiogenic activity of these compounds was analyzed through the assays in chorioallantoic membrane (CAM) of chick embryo and dorsal skin vascularization in mice, but before was realized a cell viability assay with human umbilical veins endothelial cells (HUVEC). Results: In the viability assay, concentrations tested between 30 and 3000 μg/ml showed a reduction of viable HUVEC number. In the CAM assay, CS and GS in an amount 2.0 mg/implant increased the vessels number as compared to control (phosphate buffered saline-PBS). In the assay of the dorsal skin vascularization of adult Swiss mice, the groups treated with CS (2 mg/implant; Gelfoam plug) exhibited an increase in the vessels number into plugs (0.52 ± 0.08 g/dl.. View More»
  DOI: 10.4172/2168-958X.1000129

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