Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Romy K Scheerle
Germany
Research Article
Achroma Software-High-Quality Policy in (a-)Typical Mass Spectrometric
Data Handling and Applied Functional Proteomics
Author(s): Michael Krappmann, Christine M Kaufmann, Romy K Scheerle, Johanna Grassmann and Thomas LetzelMichael Krappmann, Christine M Kaufmann, Romy K Scheerle, Johanna Grassmann and Thomas Letzel
Data evaluation of mass spectrometric raw data is an essential step to obtain high-quality results. In our days an exorbitant amount of raw data are produced in analytical (bio)chemistry due to the utilization of sophisticated experimental setups. The recently published free software Achroma has been developed to overcome increasing data processing challenges by providing the possibility of a comprehensive data analysis (http://openmasp.hswt. de/pages/project/achroma.php). To illustrate (a-)typical data evaluation, an online coupled continuous flow system hyphenated with mass spectrometric detection was applied to investigate enzymatic activity changes in the presence of regulatory molecules and alternative substrates. The extended software strategy, the processing as well as evaluation of data is presented in detail based on enzymatic assays of intestinal alkaline phosphatase (iAP) a.. View More»
DOI:
10.4172/jpb.1000328