Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
S. Patricia Becerra
Section of Protein Structure and Function, Laboratory of Retinal Cell and Molecular Biology,
NIH-NEI, LRCMBSPSF, Building 6, Room 134, 6 Center Drive MSC 0608, Bethesda, MD 20892-0608
Tanzania
Research Article
Effects of Docosahexaenoic Acid in Preventing Experimental Choroidal Neovascularization in Rodents
Author(s): Sara Moghaddam-Taaheri, Monica Agarwal, Juan Amaral, Irina Fedorova, Elvira Agrón, Norman Salem Jr., Emily Chew and S. Patricia BecerraSara Moghaddam-Taaheri, Monica Agarwal, Juan Amaral, Irina Fedorova, Elvira Agrón, Norman Salem Jr., Emily Chew and S. Patricia Becerra
Background: The purpose of this study is to evaluate the effects of docosahexaenoic acid (DHA), a major omega-3- polyunsaturated fatty acid (ω-3-PUFAs), in the development of experimental choroidal neovascularization (CNV) in rodents.
Methods: Experimental second generation Long Evans rats fed with diets of varying ω-3-PUFA content designed to produce significantly different retinal DHA levels were used in our studies. A transgenic mouse model (fat-1) engineered to over-produce DHA was also studied. CNV was induced by rupture of Bruch\'s membrane using laser photocoagulation. At 7 days after induction, animals were euthanatized, and eyes were collected. RPE/choroid flatmounts were labeled with isolectin IB4 to determine CNV lesion volumes using confocal microscopy and high-performance 3D imaging software.
Results:.. View More»