Journal of Clinical and Cellular Immunology
Open Access

Sameh Basta

Publications

• Review Article
  Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(IFNγ+LPS) or Alternatively Activated M(IL-4) Macrophages
  Author(s): Andra Banete, Paulina Achita, Katherine Harding, Rylend Mulder and Sameh BastaAndra Banete, Paulina Achita, Katherine Harding, Rylend Mulder and Sameh Basta
  
  Objective: Macrophages (Mϕ) represent a link between the innate and adaptive arms of the immune system. Generally, Mϕ are classified into two major subsets after stimulation; either ascribed classically (M1), or more specifically M(IFNγ+LPS) based on the activating condition, or alternatively (M2), or more specifically M(IL-4) activated cells. The purpose of the study was to evaluate an immortalized murine Mϕ cell line (BMA) as an in vitro model for Mϕ polarization into M(IFNγ+LPS) and M(IL-4) phenotypes to facilitate the progress in this exciting research field. Methods: The BMA cell line was stimulated with either IFNγ and LPS or IL-4 to induce cellular polarization. The cell.. View More»
  DOI: 10.4172/2155-9899.1000318

  Abstract PDF

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