Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Research Article
Diminished IL-10 production is Associated with Impaired Versatility of Monocytes in Familial Mediterranean Fever
Author(s): Tigran K. Davtyan, Gagik S. Hakobyan, Samvel A. Avetisyan, Anna G. Sukiasyan and Yuri T. AleksanyanTigran K. Davtyan, Gagik S. Hakobyan, Samvel A. Avetisyan, Anna G. Sukiasyan and Yuri T. Aleksanyan
Purpose: The nature of the heightened endotoxin sensitivity state observed in Familial Mediterranean Fever (FMF) at present remains unknown. To assess the possibility that IL-10 plays a role in setting the inflammatory threshold, we studied IL-10 production by monocytes and dendritic cells as well as endotoxin tolerance induction in FMF patients.Methods: 46 attack-free FMF patients were included in this study. The production of IL-10 by NLR- or TLRagonist- stimulated monocytes and dendritic cells were assayed either by conventional ELISA or flow cytometry. The versatility of monocytes was studied by measuring the production of IL-10 and IL-1β after stimulation by pro- and anti-inflammatory agents, and after stimulation arrest or a further counter stimulation. Monocyte endotoxin tolerance and cross-tolerance induction were assayed by measuri.. View More»
DOI:
10.4172/2155-9899.1000196