Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Research Article
Clinically Applicable Diagnostics Assay Development for Lung Cancer
Biomarker, SAA
Author(s): Hye-Jin Sung, Sang-Su Na, Keyung-Jo Seul, Hyo-Sung Jeon, Jae Yong Park and Je-Yoel ChoHye-Jin Sung, Sang-Su Na, Keyung-Jo Seul, Hyo-Sung Jeon, Jae Yong Park and Je-Yoel Cho
Background: Serum Amyloid A (SAA) is an acute phase protein and has been reported as a lung cancer biomarker. Many cancer protein biomarkers have been reported but few are currently in clinical application. This is due to the obstacles in the process from biomarker discovery to biomarker application: validation in large scale clinical samples and paired antibody production for the development of immunoassay-based diagnostics.
Methods: To develop immunoassay-based diagnostics of SAA, we produced anti-SAA monoclonal antibodies after bacterial production of pure SAA whole protein immunogen. Using two SAA specific monoclonal antibody clones, we developed two types of diagnostics; ELISA and rapid tester diagnostics. Hundreds of clinical samples were tested using the immunoassay diagnostics developed.
Results: The diagnostic or differential diagnostic ability of lung cancer .. View More»
DOI:
10.4172/jpb.1000333