Journal of Alcoholism & Drug Dependence
Open Access
ISSN: 2329-6488
ISSN: 2329-6488
Satoshi Watanabe
Japan
Research Article
Acute Ethanol Administration Increases Mir-124 Expression via Histone Acetylation in the Brain
Author(s): Keisuke Mizuo, Shunichiro Okazaki, Satoshi Watanabe, Hiromasa Inoue and Hiroshi Matsumoto
Keisuke Mizuo, Shunichiro Okazaki, Satoshi Watanabe, Hiromasa Inoue and Hiroshi Matsumoto
Acute ethanol administration causes an enhancement of mesolimbic dopaminergic activity in the brain. The microRNAs miR-124 and miR-132 are highly expressed in the central nervous system and contribute to the regulation of mesolimbic dopaminergic activity. However, the nature of a potential mechanistic association between ethanol and these microRNAs has remained unclear. Recent studies reported that the expression of some microRNAs was regulated by histone acetylation. In this study, we investigated whether ethanol administration can change the expression of miR-124 and miR-132 via histone acetylation in mouse brain. We administered 2 g/kg ethanol (s.c.) to male C57BL / 6J mice. Total RNA was extracted using TRIzol in the whole brain 1 to 12 hr after administration. MicroRNAs were detected by northern blotting. Acetylated histone H3 an.. View More»
DOI:
10.4172/2329-6488.1000232