Fisheries and Aquaculture Journal
Open Access

Satshil B Rana

Publications

• Research Article
  De Novo Assembly and Analysis of the Testes Transcriptome from the Menhaden, Bervoortia tyrannus
  Author(s): Frank J Zadlock IV, Satshil B Rana, Zain A Alvi, Ziping Zhang, Wyatt Murphy and Carolyn S Bentivegna Frank J Zadlock IV, Satshil B Rana, Zain A Alvi, Ziping Zhang, Wyatt Murphy and Carolyn S Bentivegna
  
  Background: The menhaden, Bervoortia tyrannus, is one of the most important fish within the oceanic ecosystem and a crucial species supporting major fisheries along the Atlantic and Gulf coasts. However, little is known about menhaden from a genetic aspect. The objective of this project is to apply high throughput sequencing to the testes of menhaden to provide the genetic tools required to further study their population dynamics Result: We applied Illumina Next Seq 500 technology to two different testes and used Velvet/Oases to perform the de novo assembly that resulted in the construction of 254,462 contigs. Applying BLASTX to annotate the contigs against the non-redundant protein database resulted in 46.89% matches. To validate the accuracy of the assembly, the reads were mapped back to transcripts (RMBT) with a percentage of 87.83%. To experimentally verify the .. View More»
  DOI: 10.4172/2150-3508.1000186

  Abstract PDF

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