ISSN: 2161-1025
Saumendra Nath Das
India
Research Article
Plasmid Mediated Antibiotic and Heavy Metal Co-Resistance in Bacterial Isolates from Mahananda River Water (Malda, India)
Author(s): Shyamapada Mandal*, Saumendra Nath Das, Manisha Mandal
Shyamapada Mandal*, Saumendra Nath Das, Manisha Mandal
Aims: The current investigation stands for the determination of MAR (multiple antibiotic resistances) indices of antibiotic resistant and heavy metal (HM) tolerant bacterial isolates from Mahananda river water near Malda (West Bengal state, India) and profiling of R-plasmids of the isolated bacteria. Methods: The water samples (n = 5), collected from Mahananda river water, Malda town, (India), were checked for the presence of bacterial growth in nutrient broth cultures. The pure bacteria colonies, procured from each of the nutrient broth cultures, were identified by conventional methods. The susceptibilities to antibiotics and HMs of the isolated bacteria were determined by disc diffusion and agar dilution, respectively. Plasmid curing of the resistant bacteria was done with SDS treatment, and the isolated plasmids were screened through agarose gel electrophoresis. The MAR indices for.. View More»