Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Saurabh Pandey
India
Research Article
In-silico Characterization, Structural Modelling, Docking Studies and
Phylogenetic Analysis of 5-Enolpyruvylshikimate-3-Phosphate Synthase
Gene of Oryza sativa L.
Author(s): Ubaid Yaqoob, Tanushri Kaul, Saurabh Pandey and Irshad Ahmad Nawchoo
Ubaid Yaqoob, Tanushri Kaul, Saurabh Pandey and Irshad Ahmad Nawchoo
The 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) is one of the vital enzymes of the shikimate pathway which is involved in the biosynthesis of secondary metabolites and several amino acids. The multiple sequence alignment of these EPSPS protein sequences from different plants showed conserved regions at different stretches with maximum homology in amino acid residues. We revealed the homology model of Oryza sativa EPSPS (OsEPSPS) protein using the structure of E. coli EPSPS as template. The resulting model structure was refined by PROCHECK, RAMPAGE server, ProSA, Verify3D etc. that indicated the model structure is reliable. Ramachandran plot analysis showed that conformations for 94.3% of amino acid residues are within the most favoured regions. Through motif analysis, it was revealed that a conserved EPSPS domain is uniformly found in all EPSPS proteins irres.. View More»
DOI:
10.4172/2167-0412.1000274