Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Schüttler J
Germany
Research Article
HPLC-MS/MS Based Time Course Analysis of Morphine and Morphine-6-Glucoronide in ICU Patients
Author(s): Andreas E, Peter J, Lerch M, Schüttler J and Jeleazcov CAndreas E, Peter J, Lerch M, Schüttler J and Jeleazcov C
Morphine is a widely-used opioid analgesic to treat post-operative pain in the intensive care unit. For the quantification of morphine and its metabolite Glucuronide (M6G) concentrations a sensitive and specific liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed and validated according to Food and Drug Administration (FDA) guidelines. Plasma samples were extracted with solid-phase extraction and substituted with deuterated morphine and M6G as internal standards. Separation was performed by gradient elution using UPLC-like system and analyzed by MS/MS consisting of an electrospray ionization source. The lower limit of quantification was 500 ‘‘pg/ml’’ for morphine and 50 ‘‘pg/ml’’ for M6G. Intra- and interassay precision and accuracy did not exceed ± 15%. The method was applied to a clinical study d.. View More»
DOI:
10.4172/2157-7064.1000368