Pancreatic Disorders & Therapy
Open Access

Sean M Martin

Publications

• Research Article
  Ascorbate-Induced Autophagy in Pancreatic Cancer
  Author(s): Jessemae L Welsh, Juan Du, Zita A Sibenaller, Sean M Martin, C Michael Knudson and Joseph J Cullen Jessemae L Welsh, Juan Du, Zita A Sibenaller, Sean M Martin, C Michael Knudson and Joseph J Cullen
  
  Pharmacological ascorbate induces autophagy in pancreatic cancer, which is characterized by accumulation of autophagosomes and the processing of microtubule-associated protein light chain 3 (LC3) to the lipidated form, LC3- II. Prior studies have demonstrated LC3-II upregulation in pancreatic cancer cells after gemcitabine chemotherapy or ionizing radiation. The aim of this study was to determine the role of autophagy in pharmacological ascorbate-induced cytotoxicity. Pancreatic cancer cells were generated to stably overexpressLC3 fused to a green fluorescent protein (MIA PaCa-2 LC3-GFP). MIA PaCa-2 LC3-GFP cells had a higher LC3-II/LC3-I ratio at 6 and 24 hours and demonstrated a clonogenic survival advantage after pharmacological ascorbate treatment compared to parental cells. In cells with knockdown of Atg5, there was increased susceptibility to pharmacological ascorbate-induced cy.. View More»
  DOI: 10.4172/2165-7092.1000119

  Abstract PDF

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