Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Shailendra Giri
Tanzania
Research Article
MicroRNA Regulation of Proinflammatory Response in X-linked Adrenoleukodystrophy
Author(s): Jaspreet Singh, Hamid Suhail and Shailendra Giri
Jaspreet Singh, Hamid Suhail and Shailendra Giri
The underlying mechanism(s) for development of the inflammatory response in inherited, fatal neurometabolic disease X-linked adrenoleukodystrophy (X-ALD) remain completely unknown. Genetic defect (ABCD1 mutation/ deletion), common to all phenotypes of X-ALD, has failed to explain the development of inflammation only in a subset of patients. In this study we document the novel role of microRNAs (miRNAs) in the development of the inflammatory response in unstimulated ALD patient-derived lymphocytes and Abcd1-knockout (Abcd1-KO) mice mixed glial cells. The levels of proinflammatory cytokine gene expression (inducible nitric oxide synthase [iNOS]) were increased in X-ALD patient-derived lymphocytes. Predictions via the use of online bioinformatics algorithms and confirmed by using miRNA mimic of inhibitor-transfection method (gain- and loss-of-function) revealed the role of miR-323-5p in .. View More»
DOI:
10.4172/2155-9899.1000349