Journal of Chromatography & Separation Techniques
Open Access

Sharaf El-Din M

Publications

• Research Article
  Stability-indicating HPLC Method for the Determination of Atenolol in Pharmaceutical Preparations
  Author(s): Belal F, Sharaf El-Din M, Aly F, Hefnawy M and El-Awady M Belal F, Sharaf El-Din M, Aly F, Hefnawy M and El-Awady M
  
  A stability-indicating reversed-phase liquid chromatographic method for the determination of atenolol was developed. Different chromatographic conditions were carefully studied to optimize the parameters for the evaluation of the studied drug. The chromatographic assay involved the use of a C8 Column (250 mm×4.6 mm i.d., 5 μm) with a simple mobile phase composed of acetonitrile:methanol:0.02 M phosphate buffer, pH 5 (20:20:60) at a flow rate of 1 ml/min and UV detection at 226 nm. Pindolol was used as an internal standard. The method showed good linearity over the range of 0.05-10 μg/mL with a detection limit of 0.01 μg/mL and a quantitation limit of 0.03 μg/mL. The proposed method was successfully applied for the analysis of atenolol in three commercial tablets with average percent recoveries of 100.14 ± 1.04, 100.20 ± 0.92, 100.00 ± 0.91 and 10.. View More»
  DOI: 10.4172/2157-7064.1000164

  Abstract PDF

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