Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

Sharon Zeligson

Publications

• Research Article
  Array-Comparative-Genomic-Hybridization (Acgh) Based Preimplantation-Genetic-Diagnosis (PGD) for Balanced Translocation Carriers Improves both Diagnostic and Pregnancy Rates Compared to Fluorescent-In-Situ-Hybridization (FISH) and Polymerase-Chain-Re
  
  Author(s): Tali Mishael, Talia Eldar-Geva1, Sharon Zeligson, Rachel Beeri, David A Zeevi, Paul Renbaum, Orit Lobel, Yafa Nevo, Raphael Ron-El, Ehud J Margalioth, Ephrat Levy-Lahad and Gheona Altarescu Tali Mishael, Talia Eldar-Geva1, Sharon Zeligson, Rachel Beeri, David A Zeevi, Paul Renbaum, Orit Lobel, Yafa Nevo, Raphael Ron-El, Ehud J Margalioth, Ephrat Levy-Lahad and Gheona Altarescu
  
  Introduction: Preimplantation genetic diagnosis (PGD) of balanced translocations is usually performed using Fluorescence-in-Situ-Hybridization (FISH), and more rarely by Polymerase-Chain-Reaction (PCR). These methods assess only chromosomes involved in the translocation, as opposed to array comparative-genomic-hybridization (aCGH) which screens all chromosomes. Material and Methods: In this report, we compared the diagnosis and pregnancy rates for balanced translocations using all three techniques in a retrospective study of 75 PGD cycles. Results: Of 331 embryos analyzed by FISH, 225 (68%) were successfully diagnosed, 34 (15%) were transferable yielding a pregnancy rate of 13% per Ovum-Pick-Up (OPU). With PCR, 80 out of 124 embryos (65%) were diagnosed, 21 (26%) were transferable, and a pregnancy rate of 14.3% per.. View More»
  

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