Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Shazi Shakil
India
Research Article
Interaction of 2009 CTX - M Variants with Drugs and Inhibitors: a Molecular Modeling and Docking Study
Author(s): Shazi Shakil and Asad Ullah KhanShazi Shakil and Asad Ullah Khan
Extended-spectrum- β -lactamases (ESBLs) are enzymes produced by bacteria which impart resistance against advanced-generation-cephalosporins. CTX-M enzymes have become the most prevalent ESBLs. The amino acid residues through which 2009 CTX-M-variants interact with drugs and inhibitors is not reported. Homology models for CTX-M-15 (This study), CTX-M-53, CTX-M-71, CTX-M-82 and CTX-M-89 were prepared. Ramachandran-Z-scores for models were found to be -0.449, 0.006, -0.103, -0.007 and 0.092, respectively. These models were docked with target drugs (cefotaxime, ceftazidime, cefepime) and inhibitors (clavulanate, sulbactam, tazobactam). The bla CTX-M-15 marker was PCR-ampli fi ed from plasmid DNA of clinical Escherichia coli isolate. Minimum inhibitory concentrations (MICs) for drugs were tested .. View More»
DOI:
10.4172/jpb.1000131